Fig. 1 | Communications Biology

Fig. 1

From: PIWI genes and piRNAs are ubiquitously expressed in mollusks and show patterns of lineage-specific adaptation

Fig. 1

Evolution and expression of PIWI genes in mollusks. a PIWI gene tree reconstruction of molluskan PIWI genes. b Control PCR with PIWI paralog specific primers and L. stagnalis cDNA from the reproductive tract. The complete gel is shown in Supplemental Fig. 1g. c qPCR results for PIWI paralog expression in different tissues of L. stagnalis, measured as n-fold expression of the housekeeping gene GPI. Center line indicates median, box limits represent the 50th percentile, whiskers show the upper and lower extremes. d PIWI paralog expression in different tissues of L. stagnalis, normalized by the expression of the housekeeping gene GPI, values from reproductive tract set to 1. Center line indicates median, box limits represent the 50th percentile, whiskers show the upper and lower extremes. e Control PCR with PIWI paralog specific primers and C. gigas cDNA from the adductor muscle. The complete gel is shown in Supplemental Fig. 1h. f qPCR results for PIWI paralog expression in different tissues of C. gigas, measured as n-fold expression of the housekeeping gene PPIA. Center line indicates median, box limits represent the 50th percentile, whiskers show the upper and lower extremes. g PIWI paralog expression in different tissues of C. gigas, normalized by the expression of the housekeeping gene PPIA, values from male gonad set to 1. Center line indicates median, box limits represent the 50th percentile, whiskers show the upper and lower extremes

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