Fig. 2

Characterization of small RNAs from L. stagnalis (foot-) muscle and reproductive tract. a Sequence read length distribution of mapped (top) and unannotated (intergenic) reads (bottom). b Results from small RNA annotation with unitas (top) and transposon content of intergenic reads (bottom). c Ping-pong signature. P-values are deduced from the corresponding Z-scores. P-values for all reads and reads that match mRNA are shown. d Differential expression of 307 predicted piRNA clusters. Colors refer to expression relative to highest/lowest expression within one tissue. Dots indicate n-fold expression of a given cluster in reproductive tract relative to muscle. e Amount of clustered reads and ping-pong reads per million bootstrapped reads (ppr-mbr). f Representation of transposons in the genome of L. stagnalis, plotted by divergence [%] from transposon consensus. g Representation of transposons within piRNA clusters of L. stagnalis, plotted by divergence [%] from transposon consensus. h Prominent transposons that are enriched or depleted in L. stagnalis piRNA clusters