Fig. 5
Confirmation of amino acid positions that control the divergent cellular responses to IgG1 and IgG4 Fc hexamers using single mutants. a Inhibition of phagocytosis by IgG1 Fc hexamer single mutants (20 μg/ml). % of IgG1 activity was calculated as (mutant % block/IgG1 WT % block in same experiment) × 100%. Data represent the individual donor response and mean of n = 1–9 donors. b Inhibition of phagocytosis by IgG1 Fc hexamer L234F. Data represent the mean ± SEM of n = 9 donors. c Inhibition of phagocytosis by IgG4 Fc hexamer single mutants (20 μg/ml). % of IgG4 activity was calculated as (mutant % block/IgG4 WT % block in same experiment) × 100%. Data represent individual donor response and mean of n = 1–4 donors. d Inhibition of phagocytosis by IgG4 Fc hexamer F234L. Data represent the mean ± SEM of n = 4 donors. e IgG1 Fc hexamer single mutant (50/33.3 μg/ml) activity in WB cytokine release. % of IgG1 IFN-γ release was calculated as (mutant IFN-γ release/IgG1 WT IFN-γ release in same experiment) × 100%. Data represent individual donor response and mean of n = 2–6 donors. f Whole-blood cytokine release by IgG1 Fc hexamer L234F. Data represent the mean ± SEM of n = 3 donors. g Whole-blood cytokine release by IgG1 Fc hexamer A327G. Data represent the mean ± SEM of n = 3 donors. h Platelet activation by IgG1 Fc hexamer L234F. Data represent the mean ± SEM of n = 3 donors. i Platelet activation by IgG1 Fc hexamer A327G. Data represent the mean ± SD of n = 2 donors
