Fig. 6 | Communications Biology

Fig. 6

From: Engineered hexavalent Fc proteins with enhanced Fc-gamma receptor avidity provide insights into immune-complex interactions

Fig. 6

Induction of the complement cascade by IgG1 Fc hexamer is ablated by the P331S mutation. Fluid-phase C4d (a), C3a (b), and sC5b-9 (c) were measured in human serum after 1 h incubation with the indicated human IgG Fc constructs (100 μg/ml) or heat-aggregated IgG (HAG) controls. Graphs show the individual donor data (n = 3) with mean ± SEM. Statistical analysis was carried out by two-way ANOVA (donors and stimulation) and Tukey’s multiple comparison test. PBS vs IgG1 Fc hexamer stimulation p < 0.0001 for C4d and C3a, n.s. for sC5b-9. IgG1 Fc hexamer vs P331S mutant p < 0.0001 for C4d and C3a, n.s. for sC5b-9

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