Fig. 5

Increased miR-96 upon depletion of BMPR2 promotes attenuation of RAD51. a Pulmonary microvascular endothelial cells (PMVECs) were transfected with control (Con) or BMPR2 siRNAs for 48 h, followed by quantitation of miR-96 (n = 5) and miR-21 (n = 3) relative to U6 snRNA measured by quantitative PCR analysis. b PMVECs were treated with vehicle (DMSO, Con) or LDN193189 (LDN; 100 nM) for 72 h followed by quantitation of miR-96 relative to U6 snRNA (n = 3). c RAD51 and BMPR2 protein in PMVECs co-transfected with Con or BMPR2 siRNAs with anti-miR-96 or control anti-miRNA. d PMVECs were transfected with control mimic (Con) or miR-96 mimic (miR-96) for 48 h, followed by quantitation of RAD51 by immunoblot (n = 3) and e the amount of DNA damage was measured using alkaline comet assay from 204–235 cells using ImageJ software. Bars represent mean ± SEM from five different experiments per conditions in (a) (miR-96) and from three different experiments per conditions in (a) (miR-21) and (b–e). *P < 0.05, **P < 0.01, and ****P < 0.001 versus respective control. Unpaired two-tailed t-test was used in (a, b) and (d–e). One-way ANOVA followed by Tukey’s multiple comparisons test was used in (c)