Fig. 2 | Communications Biology

Fig. 2

From: A minimal helical-hairpin motif provides molecular-level insights into misfolding and pharmacological rescue of CFTR

Fig. 2

Reversal of V232D TM3/4 hairpin opening by Lumacaftor. a Structure of the pharmacological corrector Lumacaftor (VX-809). b FRET efficiency histograms of V232D TM3/4 (chartreuse) in POPC vesicles at increasing concentrations of VX-809. PDA fits are shown as red cityscapes. c Dose–response curve of VX-809 action on V232D TM3/4 (chartreuse). Depicted is the fraction of compact hairpin conformation as a function of corrector concentration. The apparent affinity was determined by a sigmoid fit (red), which yielded an EC50 of ~350 µM. Other fit parameters were fF,min = 0.24, fF,max = 0.87, and n = 0.91. Errors in c are standard deviations of the PDA chi-square minimization algorithm calculated from ten iterations

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