Fig. 1 | Communications Biology

Fig. 1

From: 2D polarization imaging as a low-cost fluorescence method to detect α-synuclein aggregation ex vivo in models of Parkinson’s disease

Fig. 1

Definition of energy funneling efficiency ε (top panel) and calculations of the fluorescence anisotropy r and ε as functions of the distance between GFP molecules in a cubic lattice (bottom panel). The homo-FRET Förster radius for GFP is 4.7 nm according to its spectral properties. On the right column pictograms of: a monomer, b dimer, c densely, and d loosely packed aggregates of α-syn-GFP. The monomer/dimer ratio indicates how many sites of the lattice are occupied by a monomer/dimer. Black arrows show the transition from the pure monomer to the pure dimer case. Error bars represent the standard deviation of the simulations when repeated 10 times

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