Fig. 2
In vitro vessel-like network elongation. a Representative confocal images of scaffolds embedded with different cell combinations, imaged 4 days post seeding. EC endothelial cells, SMC smooth muscle cells. Endothelial cell-ZsGreen (naive or ANGPT1-secreting) are seen in green; scale bar = 100 µm. b Percentage of elongated elements with an eccentricity score of 0.95–1, 4 days post seeding. Myo myoblasts. Data are expressed as box-and-whisker plots, where the central lines denote medians, edges represent upper and lower quartiles and whiskers show minimum and maximum values. Data were analyzed by one-way ANOVA, followed by Holm–Sidak’s multiple comparison test, n = 6 (*p = 0.0336 versus EC-SMC co-culture, ##p = 0.0026 versus EC-SMC-Myo tri-culture, **p = 0.0027 versus EC-SMC co-culture, ****p < 0.0001 versus EC-SMC co-culture). c Percentage of elongated elements with an eccentricity score of 0.95–1, 7 days post seeding. Data are expressed as box-and-whisker plots, where the central lines denote medians, edges represent upper and lower quartiles and whiskers show minimum and maximum values. Data were analyzed by one-way ANOVA, followed by Holm–Sidak’s multiple comparison test, n = 6 (*p = 0.049 for ECANGPT1-SMC versus EC-SMC co-culture, *p = 0.0316 for ECANGPT1-SMCs versus EC-SMCVEGF co-culture, *p = 0.0477 for ECANGPT1-SMCVEGF-Myo tri-culture versus EC-SMC co-culture, #p = 0.0475 versus EC-SMC-Myo tri-culture)
