Fig. 7
New muscle formation in vivo. a Representative images of Masson’s trichrome-stained naive tri-culture-embedded scaffolds 14 days post transplantation. Newly formed muscle bundles are seen in light purple. Scale bar = 500 µm. b Magnification of the red rectangle in (a). Scale bar = 100 µm. c Representative images of Masson’s trichrome-stained ANGPT1- and VEGF-secreting tri-culture-embedded scaffolds 14 days post transplantation. Newly formed muscle bundles are seen in light purple and a mature muscle in black is indicated by a yellow arrow. Scale bar = 500 µm. d Magnification of the red rectangle in (c). Scale bar = 100 µm. e Representative immunofluorescent signals of stained cryo-sections. Blue indicates desmin-positive staining, magenta mouse CD31-positive staining. Scale bar = 100 µm. f New myofiber area quantification. Data are expressed as box-and-whisker plots, where the central lines denote medians, edges represent upper and lower quartiles and whiskers show minimum and maximum values. Data analyzed by unpaired two-tailed Student’s t-test, n = 6 (*p = 0.0370). g Myofiber area quantification, as determined by desmin staining. Data are expressed as box-and-whisker plots, where the central lines denote medians, edges represent upper and lower quartiles and whiskers show minimum and maximum values. Data analyzed unpaired two-tailed Student’s t-test, n ≥ 4 (*p = 0.0268)
