Fig. 3 | Communications Biology

Fig. 3

From: Activation of oxytocin neurons in the paraventricular nucleus drives cardiac sympathetic nerve activation following myocardial infarction in rats

Fig. 3

Parvocellular PVN vasopressin neuronal activation following acute MI. Representative photomicrographs of coronal sections through the brain at the level of the PVN following a sham or b acute myocardial infarction (MI). Black staining indicates c-Fos-positive nuclei, brown staining indicates vasopressin-positive cytoplasm, and black nuclei surrounded by brown staining indicates co-localization of c-Fos protein with vasopressin (yellow arrows). Insets a, b High-power magnification of the dashed-boxes taken from the low magnification micrographs. The bar graphs (mean ± SEM) show the number of: c c-Fos-positive cells, d vasopressin-positive cells, e cells co-localized with vasopressin+c-Fos-protein, and f percentage of oxytocin-positive cells expressing c-Fos protein per section in bilateral parvocellular division of the PVN. **P < 0.01 significant difference between MI rats (n = 8) and sham rats (n = 8; unpaired t-test). Scale bars = 50 μm. 3V third ventricle, pPVN parvocellular paraventricular nucleus

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