Fig. 1
The rDNA-stabilizing Pbp1 paradoxically promotes Ty1 retrotransposition. a Transcription of a Ty1 element/gene (1), translation (2), cDNA synthesis inside virus-like particles (3), and hotspot integration (4). Larger circles = Ty1 Gag protein; Small circles/squares = Ty1 protease, integrase, and reverse-transcriptase; Horseshoe = Ty1 cDNA. b Schematic illustrating Ty1his3AI retromobility assay. His− cells become His+ only after a complete retrotransposition event has occurred with splicing of the artificial intron. c Effect of pbp1∆ on retromobility of Ty1his3AI. d Schematic illustrates Ty1 integration PCR assay. Ty1 cDNA integrates upstream of RNA Pol III-transcribed genes. PCR primers are indicated with arrows. e Semi-quantitative PCR products reflecting Ty1 integration upstream of 12 tRNAGLY loci and one tRNATYR gene. f Effects of lsm12∆ and pbp4∆ on retromobility of Ty1his3AI. g Semi-quantitative PCR products reflecting Ty1 integration upstream of 12 tRNAGLY loci. h Frequency of Ty1his3AI retromobility in a Pbp1 truncation mutant lacking the C-terminal Pab-1-binding domain (also see Supplementary Fig. 1). i Semi-quantitative PCR products reflecting Ty1 integration upstream of 12 tRNAGLY loci. c, f, h Mean ± SD; n = 5 independent cultures; Mann–Whitney U-test. e, g, i TEL1 amplification served as control. a–i *p < 0.05; **p < 0.01
