Fig. 3 | Communications Biology

Fig. 3

From: Conserved Pbp1/Ataxin-2 regulates retrotransposon activity and connects polyglutamine expansion-driven protein aggregation to lifespan-controlling rDNA repeats

Fig. 3

Humanization of yeast Pbp1 with neurodegeneration-linked polyQ expansion. a Schematic of Pbp1-polyQ expansions generated in yeast to reflect ALS/SCA2 human genetics. b Effects of Pbp1-polyQ expansion on the level of Pbp1 transcripts. Shown are Pbp1 mRNA levels as detected by RT-qPCR (Mean ± SD; n = 3). Values and statistics are relative to levels in Pbp1-1Q cells (one-way ANOVA and Dunnett’s post hoc; ****p < 0.0001). c Effects of Pbp1-polyQ expansions on Pbp1 protein levels. Levels of Pbp1-TAP were detected by western blotting. d–f CoIP examining interactions between Pbp1-polyQ and Lsm12 d, Pbp4 e, and Pab1 f

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