Fig. 2 | Communications Biology

Fig. 2

From: RanBP2 regulates the anti-retroviral activity of TRIM5α by SUMOylation at a predicted phosphorylated SUMOylation motif

Fig. 2

RanBP2 promotes TRIM5α SUMOylation within a TRIM5α/RanGAP1/RanBP2/Ubc9 complex. HeLa cells were transduced with a lentiviral vector coding for a shRNA against RanBP2 (shBP2), shTRIM5α, empty vector (shE), or not transduced (NT). After 3 days, cells were lysed and immunoprecipitated with a α-RanGAP1 or b α-TRIM5α antibodies. Input samples were analyzed by immunoblotting with a α-RanBP2, α-TRIM5α, α-Ubc9, and α-Actin antibodies, while immunoprecipitates were probed with α-Ubc9, α-RanBP2, α-TRIM5α, or α-SUMO1 antibodies. Higher molecular weight bands indicate SUMO-modified TRIM5α (TRIM5α*S1). Western blots are representative of 2 and 3 independent experiments, respectively (original blots in Supplementary Figures 3)

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