Table 1 Resulting contig and scaffold N50s are presented here for each major step in the process of assembling EquCab3

From: Improved reference genome for the domestic horse increases assembly contiguity and composition

Sequence composition

Contig N50

Scaffold N50

EquCab3

  

Sanger + MaSuRCa super reads

1.2 Mb

6.6 Mb

Sanger + MaSuRCa super reads + Chicago + Hi-C

1.2 Mb

86 Mb

Sanger + MaSuRCa super reads + Chicago + Hi-C + PacBio

4.5 Mb

86 Mb

EquCab2

  

Sanger Fosmid + BAC + Radiation Hybrid Map data

112 kb

46 Mb

  1. For comparison, the contig and scaffold N50s for the final EquCab2 product are also shown. The final EquCab3 product (Sanger + MaSuRCa super reads + Chicago + Hi-C + PacBio) improved the contig N50 40-fold, and the Scaffold N50 was improved from a chromosome arm-limited 46 Mb to a chromosome length-limited 86 Mb
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