Fig. 2

Quantitative analysis of the adhesion dynamics. The individual velocity and amplitude trajectories were parameterized and the following parameters were obtained: a the mean translational velocity, v; b fluorescence intensity amplitude difference, ΔA, (a.u., arbitrary units); c contact time; and d Pearson correlation coefficient between fluorescence amplitude and velocity profile. Note that the data for parasitized HbAA and HbAS erythrocytes are statistically different, according to an F-test (trophozoites: F = 58, DF = 21, p < 0.001; schizonts: F = 29, DF = 21, p < 0.001). Each data point corresponds to a single cell measurement, with at least 27 cells being measured per condition. A box plot analysis is overlaid over the individual data points, with the median, 25% and 75% quartile ranges and the standard error of the mean being shown. Statistical significance was assessed, using Holm–Sidak one-way ANOVA (velocity) or Dunn’s ANOVA on ranks (amplitude and contact time). Wall shear stress, 0.03 Pa