Fig. 6
Hypoxia increases E2F3d transcripts. a HeLa cells were cultured under normoxic (21% O2) or hypoxic (1% O2) conditions for 24 h. cDNA samples were subjected to qRT-PCR using primer sets specific for each E2F3 member and HIF-1 target genes. Values shown represent the means of three independent experiments. Error bars represent SD. **P < 0.01. n.s. not significant. b HeLa cells were co-transfected with an E2F3a promoter (−740 to +160) luciferase reporter plasmid and pCMV-RL. After 24 h of transfection, cells were cultured for a further 24 h under normoxic or hypoxic conditions. Values shown represent the means of three independent experiments. Error bars represent SD. **P < 0.01. c Model for E2F3d-mediated mitophagy for mitochondrial quality control in cancer cells
