Fig. 1
From: Beclin 1 regulates recycling endosome and is required for skin development in mice
Phenotypes of Beclin 1 cKO mice. a Expression of Beclin 1 in the dorsal skin of E18.5 embryos of the indicated mice. Actin was used as a loading control. Arrow indicates the band of Beclin 1. Asterisk indicates a nonspecific band. Representative images of five independent experiments are shown. b Gross appearance of Beclin 1 cKO neonatal mice is shown. Scale bars = 1 cm. c TEWL assay of neonatal mice. High TEWL values indicate water loss from the epidermis (n = 8, mean ± SD). Asterisk indicates a significant difference at p < 0.0001. (Student’s t test) d Body weight loss in Beclin 1 cKO neonatal mice (n = 4, mean ± SD). Asterisks indicate a significant difference at p < 0.0001. (ANOVA) e Loss of skin barrier function in the epidermis of Beclin 1 cKO neonates. Toluidine Blue permeability assay showing the blue staining of a Beclin 1 cKO neonate, which shows impaired barrier function. Scale bars = 1 cm. Uncropped immunoblot images are provided in Supplementary Figure 4a. Data sources for c and d are provided in Supplementary Data 1
