Fig. 3
From: Beclin 1 regulates recycling endosome and is required for skin development in mice
Unbalanced spindle orientation in basal keratinocytes of Beclin 1 cKO mice. a Representative images of symmetric (top) and asymmetric (bottom) division in the dorsal skin of E17.5 embryos of wild-type mice. Dividing basal cells were immunostained with an anti-survivin antibody (red). White dotted lines indicate the basal membrane. Scale bars = 10 µm. b Angles of spindle orientation relative to the basal membrane were analyzed in multiple fields and shown graphically (n = 8). c Percentage of basal cell division in the epidermis of Beclin 1 cKO and control mice. The direction of division was classified into three types (parallel: 0–30°, perpendicular: 60–90°, and others: 30–60°) according to the angle from the basal membrane. Data sources for b and c are provided in Supplementary Data 2
