Fig. 4 | Communications Biology

Fig. 4

From: CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

Fig. 4

Sequence analysis of genome-edited plants of Gonja Manjaya to detect targeted mutations in S3 target site. Nucleotides in brown represent the target site and blue nucleotides indicate PAM. Dotted lines (…) represent deletions, nucleotides in red indicate insertion. BSOLV, sequence of BSV strain Obino l’Ewai; WT, wild type non-edited control Gonja Manjaya; 1‒100, independent transgenic events. The number of indels as deletions (−) or insertions (+) and percentage of clones of transgenic event showing same mutations are indicated on the right side of the figure. The PCR products amplified using primer set P3 (see Fig. 1b) were purified, cloned, and 10 individual clones per event were analyzed for mutations at target site S3 by Sanger sequencing

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