Fig. 6
From: Tasmanian devils with contagious cancer exhibit a constricted T-cell repertoire diversity
Relative quantitative PCR analysis of gene expression in DFTD and non-DFTD devils. a Fold change in the expression level of nine T-cell marker, transcription factor or cytokine genes in DFTD devils in comparison with non-DFTD devils (log-2 scale). Box-plot elements: centre line, median; box limits, first and third quartiles; whiskers, minimum and maximum ranges. b Association between T-bet and IFN-γ gene expression, and between GATA-3 and IL-4 expression in wild devils. Gene expression was normalised to two reference genes, GAPDH and GUSB. Relative expression of GUSB normalised to GAPDH is shown (in panel a) as a control for non-differentiated expression between DFTD and non-DFTD samples
