Fig. 4 | Communications Biology

Fig. 4

From: Mutations in the PKM2 exon-10 region are associated with reduced allostery and increased nuclear translocation

Fig. 4

PKM2 exon-10 variants show increased interaction with KDM8. a, b Co-immunoprecipitation (IP) assay was conducted by co-transfecting MCF7 cells with Flag-KDM8 plus a HA-tagged PKM2 (HA-PKM2) protein as indicated, followed by IP with anti-HA (a) or anti-Flag (b) and Western blotting analysis. c IP assay was conducted by transfecting MCF7 cells with a HA-PKM2 as indicated, followed by IP with anti-KDM8 and the subsequent Western blotting analysis. d GST pull-down assays were performed with GST or GST-KDM8 and a His-tagged PKM2 (His-PKM2) protein as indicated. WB, Western blotting analysis; EV, empty vector; endo., endogenous; CBB, Coomassie brilliant blue; WT, wild-type

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