Fig. 1
From: Activated cofilin exacerbates tau pathology by impairing tau-mediated microtubule dynamics
Cofilin reduction rescues the deficit in tau–tubulin binding in APP/PS1 mice. a Confocal images of tubulin–tau PLA staining in the cortex from 7-month-old WT, APP/PS1, and APP/PS1;cofilin+/− littermates showing significantly decreased tubulin–tau PLA signals in APP/PS1 mice (scale bar = 20 μm). b, c Quantification of tubulin–tau PLA intensity and total area. Data are expressed as mean ± SEM (one-way ANOVA with Tukey post hoc, n = 4/genotype, #p < 0.0001). d Confocal images of tubulin–cofilin PLA staining in the cortex from 7-month-old WT, APP/PS1, and APP/PS1;cofilin+/− littermates showing markedly increased tubulin–cofilin PLA signals in APP/PS1 mice. mice (scale bar = 20 μm). e, f Quantification of tubulin–cofilin PLA intensity and total area. Data are expressed as mean ± SEM (one-way ANOVA with Tukey post hoc, n = 4/genotype, #p < 0.0001). g Increased tubulin–tau complexes in APP/PS1;cofilin+/− compared to APP/PS1 cortical primary neurons by co-IP. h Quantification of tubulin–tau. Data are expressed as mean ± SEM (one-way ANOVA with Tukey post hoc, n = 4/genotype, *p = 0.0119, **p = 0.0018. i Quantification of tubulin–cofilin complexes. Data are expressed as mean ± SEM (one-way ANOVA with Tukey post hoc, n = 4/genotype, *p = 0.0359, **p = 0.0095)
