Fig. 1
From: Differences in glutamate uptake between cortical regions impact neuronal NMDA receptor activation
Astrocytic glutamate uptake is facilitated in the ACC during high-frequency synaptic stimulation. a, b Schemes representing the astrocytic whole-cell patch-clamp recordings in layer 1 of the barrel cortex (a) and in layer 1 of the ACC (b) in acute brain slices. Currents were evoked by focal electrical stimulation via a theta-glass pipette placed in layer 1 in proximity to the recorded astrocyte. c Representative traces of the inward current evoked in an astrocyte by a single pulse stimulation in the barrel cortex (left) and in the ACC (right). d The average decay time of the STCs elicited by a single stimulation in the barrel cortex (τdecay = 3.5 ± 0.13 ms; n = 12) is similar to that in the ACC (τdecay = 3.45 ± 0.13 ms; n = 15). e Superimposed representative traces of the inward current evoked in an astrocyte by an extracellular stimulation train of 11 pulses (black) and a train of 10 pulses (gray) at 50 Hz and 100 Hz in the barrel cortex. f In the barrel cortex, the average STCs decay time elicited by the 11th pulse of 50 Hz trains is significantly faster than that elicited by the 11th pulse of 100 Hz trains (n = 12, N = 7, *P = 0.0162) each point represents the STC decay time in one astrocyte at 50 Hz (light blue) then at 100 Hz (dark blue). g Same as (e) for the anterior cingulate cortex. h In the ACC, the average STCs decay time elicited by the 11th pulse of 50 Hz trains is significantly slower than that elicited by the 11th pulse of 100 Hz trains (n = 15, N = 6, ***P < 0.0001). Each point represents the STC decay time of one astrocyte at 50 Hz (pink) then at 100 Hz (purple). Representative traces are the average of at least five sweeps. n = number of cells, N = number of mice. Data are mean ± SEM. Two-tailed paired t test
