Fig. 3
From: Differences in glutamate uptake between cortical regions impact neuronal NMDA receptor activation
Exogenous glutamate is scavenged differently in the BC and in the ACC. a Left: cartoon depicting localized glutamate puff (1 mM) through a pipette in layer 1 of the barrel cortex. Right: example traces of the glutamate puff-evoked iGluSnFr responses triggered by ten short (8 ms) puffs at 50 Hz (light blue) and 100 Hz (dark blue) in the BC. b The decay of the glutamate puff-evoked iGluSnFr responses in the BC upon 100 Hz stimulation is slower compared with 50 Hz (n = 9, ***P = 0.0001). c Left: same as a but in the ACC. Right: Example traces of the glutamate puff-evoked iGluSnFr responses triggered by ten short puffs at 50 Hz (light pink) and 100 Hz (dark pink) in the ACC. d The decay of the glutamate puff-evoked iGluSnFr responses in the ACC upon 100 Hz stimulation is faster compared with 50 Hz (n = 10, ***P = 0.0009). e Example traces of glutamate puff-evoked iGluSnFr responses triggered by continuous 100 ms in the BC and in the ACC. f The decay of glutamate puff-evoked iGluSnFr responses by 100 ms stimulations was slightly but not significantly slower in the BC compared with the ACC (BC: n = 13, ACC: n = 18, P = 0.059). g Example traces of 200 ms glutamate puffs in the BC and in the ACC. h Upon continuous 200 ms glutamate puffs, the decay of iGluSnFr evoked signals were significantly slower in the BC compared with the ACC (BC: n = 13, NBC = 3, ACC: n = 18, NACC = 3, **P = 0.0053). Traces normalized to the peak. n = number of slices, N = number of mice. Data are mean ± SEM. Two-tailed paired t test and two-tailed unpaired t test
