Fig. 4
From: Differences in glutamate uptake between cortical regions impact neuronal NMDA receptor activation
NMDA EPSCs in layer 5 pyramidal neurons are faster following high-frequency stimulations in the ACC. a Image of patch-clamp recording from soma of a biocytin-labeled layer 5 (L5) pyramidal cell in the ACC showing the location of the recording and stimulation pipettes (layer 5 and inner layer 1, respectively). Scale bar = 100 μm. b Representative traces of NMDA-evoked EPSCs in soma of L5 pyramidal cells in the ACC following single stimulation, trains of 10 stimuli at 50 and 100 Hz (holding potential at + 40 mV). The decay kinetics of NMDA-evoked EPSCs are faster following trains of 100 Hz stimulation than following 50 Hz stimulation (n = 9, N = 6, **P = 0.005). c Image of a biocytin-labeled L5 pyramidal cell in the BC. Whole-cell patch-clamp recordings were either acquired from the soma or from the apical dendrite. Scale bar = 100 μm. d Somatic recordings: representative traces of NMDA-evoked EPSCs in soma of L5 pyramidal cells in the BC following single stimulation, trains of 10 stimuli at 50 and 100 Hz. The decay kinetics of NMDA-evoked EPSCs in the soma of L5 pyramidal cells is slightly slower (by 4%) upon 100 Hz stimulations compared with 50 Hz stimulations (n = 9, N = 6, *P = 0.025). e Dendritic recordings: representative traces of NMDA-evoked EPSCs in the recorded dendrites. The decay kinetics of NMDA-evoked EPSCs in the dendrites of L5 pyramidal cells becomes 43.2% slower upon 100 Hz stimulations compared with 50 Hz stimulations (n = 5, N = 5, *P = 0.04). n = number of cells, N = number of mice. Data are mean ± SEM. Two-tailed paired t test
