Fig. 6
From: Differences in glutamate uptake between cortical regions impact neuronal NMDA receptor activation
Complete blockade of GLT-1 transporters reverses glutamate uptake facilitation at 100 Hz in the ACC. a Representative responses of iGluSnFr signal in the barrel cortex following synaptic stimulation (single stimulation, 10 × 50 Hz and 10 × 100 Hz) during baseline (black traces) and after application of DHK (300 μM, blue traces). b Decay kinetics of extrasynaptic glutamate transients are similarly and significantly slower in the presence of DHK (300 μM) following single pulse stimulation (n = 10, *P < 0.05), 50 Hz stimulation (n = 10, **P < 0.01) and following 100 Hz stimulation (n = 10, N = 3, **P < 0.001). c Same as (a) for the ACC. d Decay kinetics of extrasynaptic glutamate transients following single pulse and 50 Hz stimulation are not affected by the presence of DHK (n = 7, P > 0.05). However, following 100 Hz stimulation the decay kinetics become significantly slower in the presence of DHK (n = 7, N = 3, ***P < 0.0001). Traces normalized to the peak. n = number of slices, N = number of mice. Data are mean ± SEM. Two-way RM ANOVA test
