Fig. 7
From: Differences in glutamate uptake between cortical regions impact neuronal NMDA receptor activation
Effect of DHK on NMDA current kinetics in the ACC. a Representative traces of NMDA currents (layer 1 synaptic stimulation) normalized to the peak amplitude recorded from layer 5 pyramidal cells in the ACC following single pulse, trains of ten pulses at 50 and 100 Hz (extracellular synaptic stimulations) at baseline (black traces) and in the presence of the GLT-1 antagonist DHK (250 μM, purple traces). Holding potential at + 40 mV. b The decay kinetics of NMDA-evoked EPSCs are slightly but not significantly slower in the presence of DHK at single pulse (baseline: 72.3 ± 9.2 ms; DHK: 93.9 ± 9.9 ms, n = 8, P > 0.05) and 50 Hz stimulations (baseline: 259.1 ± 18.6 ms; DHK: 281.4 ± 10.1 ms, n = 9, P > 0.05). However, at 100 Hz, the decay kinetics become significantly slower in the presence of DHK (baseline: 215 ± 13.6 ms; DHK: 305 ± 9.1 ms, n = 9, N = 3, *** P < 0.001). n = number of cells, N = number of mice. Data are mean ± SEM. Two-way RM ANOVA test
