Fig. 3
Clustering Ψ response profiles of alternative spliced isoforms using WGCNA. a The inclusion levels (Ψ values) of alternative spliced isoforms calculated by MISO are clustered using WGCNA. Background colours represent the clustering of Ψ profiles for the active compounds (blue) and the control (grey) compounds, respectively (the top three clusters are shown for each compound, sorted based on cluster sizes). Horizontal and vertical axes represent inhibitor concentrations, and MISO Ψ values. Each black line illustrates the Ψ response of one MISO isoform in a given condition, and the red lines represent the consensus response in each cluster computed by averaging Ψ values of all the isoforms. b Stacked bars, showing the cumulative ratios (vertical axes) of each alternative spliced type in the set of monotonically increasing and decreasing events (horizontal axes) for each cell line:drug pair. The composition of alternative splicing types is clearly different between the two sets. c A sankey diagram illustrating the proportion of shared events between monotonic sets identified in part a. Monotonically increasing and decreasing sets are shown in red and blue rectangles, respectively
