Fig. 7

EGCG ((−)-epigallocatechin gallate), kaempferol, and quercetin are active components of hawk tea extract (HTE). a Free cholesterol uptake analysis (n = 3) and b quantitative real-time PCR (qRT-PCR) analysis of low-density lipoprotein receptor (LDLR) (n = 3) under the treatment of four potential Niemann–Pick C1-like 1 (NPC1L1) inhibitors (100 μM) in HepG2 cells. c NPC1L1 translocation analysis under EGCG treatment (50 μM) in CRL1601/NPC1L1-EGFP cells. Scale bar = 20 μm. The green fluorescence signals indicate the distribution of NPC1L1 protein. d qRT-PCR analysis of microsomal triglyceride transfer protein (MTP) and e apolipoprotein B (APOB) under the treatment of five potential hepatocyte nuclear factor 4α (HNF4α) ligands (100 μM) in HepG2 cells (n = 5). f Luciferase activity of pGL3-MTP and g pGL3-APOB promoter in exposure to five potential HNF4α ligands (100 μM) in HeLa cells (n = 3). h Representative Western blot analysis of MTP and quantitative analysis of MTP protein (n = 4) and i secretion of APOB under kaempferol (100 μM) and quercetin (100 μM) treatment in HepG2 cells (n = 4). Original Western blot images are shown as the Supplementary Fig. 16. K: kaempferol; Q: quercetin. Data are shown in mean ± SD. Statistical analyses were conducted using paired Student’s t test. *p < 0.05; **p < 0.01. Filled circles indicate the individual data points of each independent experiments under the indicated treatment conditions