Fig. 5
From: Cyclization-blocked proguanil as a strategy to improve the antimalarial activity of atovaquone
In vitro activity of proguanil and tBuPG with and without isopentenyl pyrophosphate supplementation. Synchronous ring-stage P. falciparum 3D7 parasitized erythrocytes were assayed using proguanil (c), tBuPG (d) or the control drugs clindamycin (a) and cycloguanil (b) over 96 h in [3H]-hypoxanthine uptake growth inhibition assays. Assays were carried out in RPMI 1640 parasite culture media supplemented with ( + isopentenyl pyrophosphate; blue, closed circles) or without (-isopentenyl pyrophosphate; black, closed circles) 200 µM isopentenyl pyrophosphate. In each case, data are mean ( ± SD) % inhibition of two independent experiments, each carried out in triplicate wells
