Fig. 6
From: Cyclization-blocked proguanil as a strategy to improve the antimalarial activity of atovaquone
Activity of proguanil and tBuPG in low versus supplemented p-aminobenzoic acid and folic acid media. The sensitivity of synchronous ring-stage P. falciparum 3D7 parasitized erythrocytes to control drugs cycloguanil (a) and chloroquine (b) and test compounds proguanil (c) and tBuPG (d) was assessed in 96 h dose response [3H]-hypoxanthine growth inhibition assays. Assays were performed in paired plates, one containing media without supplemented p-aminobenzoic acid and folic acid (low p-aminobenzoic acid and folic acid; black solid line) and the other with supplemented p-aminobenzoic acid and folic acid (high p-aminobenzoic acid and folic acid; 1 mg /mL each; blue dashed line). Data are presented as mean ( ± SD) % inhibition of two independent experiments, each carried out in triplicate wells
