Fig. 2
3D Large-scale localization imaging by Bessel lightsheet confinement. Super-resolution images of MC3T3-E1 cell lines with microtubule labeling (a) multiple cells plotted as maximum intensity projection over the entire imaging volume and (b–g) series of spatially sectioned sub-area enclosed by a white box illustrating the continuously optical sectioned images along the z-axis with an interval of 0.17 μm. h The Fourier ring correlation (FRC) was plotted with respect to the imaging volume used in the calculation of the image reconstruction. i–k A comparison of raw images within a sub-area (see a) enclosed in the tilt yellow box indicate the image orientation as shown in the yellow region in the inset of Fig. 1 are observed with regular lightsheet (i), localization-based lightsheet (j), and reconstructed image of the localization-based lightsheet (k). The raw image volume is composed of 192 × 672 pixels, 151 z-planes at 20 ms exposure time each, and a z-stack within a total of 3.02 s. The reconstructed image is made of 270 localization volumes in 15 min (all the scale bars: 5 µm)
