Fig. 3
Astigmatism assisted to improve axial resolution for 3D lightsheet localization imaging. Representative images of the reconstructed three-dimensional super-resolution images of primary cultured neurons. a 3D maximum intensity projections of multiple neurons shown in a large field-of-view. Scale bar is 10 µm. b Reconstructed 3D super-resolution image of neurons; the two subareas (c, d) are enlarged to show a detailed structure of the intersected tubulin network. The pseudo-color scheme represents the density within a fluorescent 100-nm diameter sphere
