Fig. 5 | Communications Biology

Fig. 5

From: Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging

Fig. 5

Live 3D localization of nucleoporins-Nup153 labeled with blinking fluorophores. a A 3D live localization of nucleoporin Nup153 expressed in HeLa cell nucleus was transfected with Halotag-EGFP-Nup153 plasmid (green overlay) and labeled with Halo-HMSiR dyes (gray overlay). Scale bar is 10 µm. b EGFP signal of Nup153 (green) is shown explicitly at four different Z slices (each were 0.5 μm apart, Z1 = 7 μm, Z2 = 7.5 μm, Z3 = 8 μm, and Z4 = 8.5 μm above coverslip) at different time points (T1 = 203.8 s, T2 = 475.6 s, T3 = 747.4 s, and T4 = 10,192.9 s). Localization results of HaloTag-labeled Nup153 proteins (gray) are accumulated from 40 time points each (T1 ± 133.6 s, T2 ± 133.6 s, T3 ± 133.6 s, and T4 = 10,192.9± 133.6 s). Scale bar is 3 µm

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