Fig. 3
From: Intravital imaging allows real-time characterization of tissue resident eosinophils
Whole-organ imaging of tissue-resident eosinophils. The organs from wild-type, eoCRE+/−/eGFP+/−, or eoCRE+/−/tdTomato+/− mice were removed and directly imaged in the green channel (a and c, ex:470 nm, em: 535 nm) or in the red channel (b and d, ex: 540 nm, em: 600 nm) using an InVivo Xtreme imaging system as described in the “Methods.” Fluorescent images are shown in color, superimposed over a black and white image of the organs. e, f Fluorescence emission signal in the green (e) and red (f) channels was quantified and corrected for background. Data represent mean ± SEM, n = 3 animals. g Skin from the dorsal flap or the ears were stained for eosinophil peroxidase and the number of eosinophils was determined as described in Fig. 1. Data represent mean ± SEM and individual values, n = 3 animals. ***p < 0.001
