Fig. 4
Saturation-transfer difference nuclear magnetic resonance (STD-NMR) experiments. a 1H-NMR spectrum of 1 mM 15d-PGJ2 and resonance assignment. b Comparison between I0 (black) and ISAT (magenta) spectra. The difference spectrum (cyan) upon 6 s irradiation confirmed the reversible interaction between hsEH CTD and 15d-PGJ2 in solution. STD values were not corrected by T1. c STD amplification factor (ASTD) vs. irradiation time plot. The plot reports the curve for four representative signals with different magnitude. d hsEH CTD—15d-PGJ2 interaction epitope mapping. The colour-map reports the percentage ASTD values as follows: yellow ≤ 33.3%; 33.4% < blue ≤ 66.6%; red > 66.7%
