Fig. 5
IC50 measurement of 15d-PGJ2 for hsEH CTD C423S/C522S (a) and WT (b). Data presented as average ± SEM of n = 6 WT and n = 4 C423S/C522S. Supplementary Fig. 7 reports the raw data used to build these IC50 curves as well as the Michaelis–Menten kinetics curves utilised to extrapolate the KM value for PHOME. This value was then used in the Cheng–Prusoff equation to obtain Ki for 15d-PGJ2, as described in the methods. KD measurements by microscale thermophoresis are reported in Supplementary Fig. 7e and f
