Fig. 2

Differential expression across two metabolic conditions. a Transcriptome-wide differential expression patterns: red indicates upregulated in glucose, blue indicates upregulated in galactose. b Gene set enrichment analysis of differentially expressed genes. The pathway most enriched is cholesterol homeostasis (upregulated in galactose condition). c Key genes involved in cholesterol biosynthesis and import are upregulated in response to the increased oxidation of glutamine that occurs in the galactose condition. Estimated FDR values are shown next to the gene names