Table 1 Contents of the embryo injection mastermix used
From: Chemical reprogramming enhances homology-directed genome editing in zebrafish embryos
Contents | Knockout mix | Knock-in mix | Knock-in mix with drugs | |||
|---|---|---|---|---|---|---|
1× (µl) | [Final] (ng/µl) | 1× (µl) | [Final] (ng/µl) | 1× (µl) | [Final] (ng/µl) | |
CRISPR sgRNA | 3.5 | 75 | 3.5 | 75 | 3.5 | 75 |
Cas9 protein | 1 | 500 | 1 | 500 | 1 | 500 |
Donor | – | – | 1 | 25 | 1 | 25 |
Drugs | – | – | – | – | 1 | Desired range |
Phenol Red | 1.5 | n/a | 0.5 | n/a | 0.5 | n/a |
Water | 3 | n/a | 3 | n/a | 2 | n/a |
