Fig. 3
From: Raftophilic rhodopsin-clusters offer stochastic platforms for G protein signalling in retinal discs
Collective behaviour of rhodopsin in disc membrane. a Representative snapshot of fluorescence imaging of a dark-adapted disc incubated with 26 nM fluorescent Fab′-1D4. Scale bar: 2 μm. b Fluorescence intensity profiles along the transverse line on a disc membrane and on the dashed line in a representative snapshot of single fluorescent spots of Fab′-1D4 (inset). Arrowheads indicate single-molecule fluorescence spots. c Kymographic presentation of dynamic heterogeneity of rhodopsin in a disc membrane. Left: pseudocolour image of a disc membrane incubated with ~20 nM fluorescent Fab′-1D4. The first frame of a movie is shown. Right: a kymograph obtained by three-dimensional-projection of a 1-s movie on ImageJ. Spatial and temporal scale bars: 2 μm and 0.1 s, respectively. d Tracking of a solitary cluster of rhodopsin. Left: a pseudocoloured snapshot of a disc membrane incubated with 9 nM fluorescent Fab′-1D4. Scale bar: 2 μm. Right: montage of consecutive images (#1–18) from movie of the square area of disc membrane (1 μm × 1 μm) in the left panel. Arrowheads indicate a transient cluster. Frame rate: 30 s−1. e Mean-square-displacement (MSD) vs. time interval (Δt) plot of rhodopsin clusters and estimated diffusion coefficient D (median ± SE; N = 18). f Histogram of cluster lifetimes. Lifetime (τ*) was obtained by exponential fitting
