Fig. 5 | Communications Biology

Fig. 5

From: Automated subset identification and characterization pipeline for multidimensional flow and mass cytometry data clustering and visualization

Fig. 5

SIC pipeline applied to mouse peritoneal cavity flow-cytometry data. a Comparison of conventional (user guided) and EPP (fully automated) clustering outcomes for wild-type (BALB/c) mouse PerC sample as displayed with MDS method (for median fluorescence values comparisons see https://figshare.com/s/9c607084d6bab0d4e1ea, 10.6084/m9.figshare.8115974). EPP clusters were annotated according to multidimensional QFMatch cluster alignment between user-guided and fully automated outcomes in the space of measured parameters (Forward Scatter, CD11b, CD11c, CD19, CD5, F4/80, IgD, and IgM). Each circle corresponds to one identified cell subset, and the size of the circle represents relative cell frequiency. Matched cell subsets are labeled with the same color. b QF-tree reflects phenotypic similarity (within the set of measured parameters) between user-guided clustering results for BALB/c mouse PerC sample. The length of edges corresponds to quadratic form score value

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