Fig. 3 | Communications Biology

Fig. 3

From: Cotton plant defence against a fungal pathogen is enhanced by expanding BLADE-ON-PETIOLE1 expression beyond lateral-organ boundaries

Fig. 3

Interaction of GhBOP1 with GhTGA3. a Yeast two-hybrid assay to detect the interaction between GhBOP1 and GhTGA1/2/3/4/5. The yeast strains containing two correct plasmids were grown on SD/–Leu/–Trp plates and SD/–Leu/–Trp/–His/–Ade plates for 4 day. b Luciferase complementation imaging analysis of the interaction of GhBOP1 with GhTGA1/2/3/4/5. Agrobacterium strains containing the indicated vector were co-expressed in N. benthamiana. The luminescent imagines were taken at 36 h after infiltration. The scale bars indicate 2 mm. c Relative Luc activities in tobacco leaves measured with luminescence intensity by IndiGo software. In present NLuc-GhBOP1, the Luc activity of the CLuc-GhTGA3 in tobacco leaves was set to 1. Error bars represent the SD (n = 12) of three biological replicates. The different letters indicate statistically different means at P < 0.05 (one-way ANOVA with a Duncan post-hoc test)

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