Fig. 8

GhBP1 repression of the expanded GhBOP1 expression beyond the lateral-organ boundary. a GhBP1 expression patterns in cotton roots at 0, 1, 2, 3, and 4 day post-inoculated with V. dahliae. GhUB7 served as an internal control. Error bars represent the SD of three biological replicates. Asterisks indicate statistically significant differences compared to corresponding expression level of mock treated plants, as performed by the Student’s t-test (*P < 0.05, **P < 0.01). b GhBP1 relative expression levels in roots of TRV:00 and TRV:GhBP1 plants. Error bars represent the SD of three biological replicates. Asterisks indicate statistically significant differences, as determined by the Student’s t-test (**P < 0.01). c GhBOP1 relative expression levels in roots of TRV:00 and TRV:GhBP1 plants. Error bars represent the SD of three biological replicates. Asterisks indicate statistically significant differences, as determined by the Student’s t-test (**P < 0.01). d GUS staining analysis of the GhBOP1pro:GUS transgenic stems with TRV:00 or TRV:GhBP1 treatment. e The disease symptoms of TRV:00 and TRV: GhBP1 plants inoculated with the V. dahliae. The photographs were taken at 18 day after V. dahliae inoculation. f The diseased rate of TRV:00 and TRV:GhBP1 cotton plants at 18 day after inoculated with the V. dahliae. Error bars represent the SD of three biological replicates. Asterisks indicate statistically significant differences compared to TRV:00, as determined by Student’s t-test (**P < 0.01). g The relative quantification of the fungal biomass in the infected stems. Error bars represent the SD of three biological replicates. Asterisks indicate statistically significant differences compared to TRV:00, as determined by Student’s t-test (**P < 0.01). h Fungal recovery assay of TRV:00 and TRV:GhBP1 plants at 18 day after V. dahliae inoculation. The stem segments at the same position of the plants were placed on PDA medium, and taken photographs at 4 day after culture