Fig. 6

Selective analysis of OlfC. a ML gene tree for OlfC subfamily 9 from all fishes. Branch with blue color indicates the foreground branch in branch-site model analysis. Numbers on the tree are bootstraps for each node with branches collapsed for low bootstrap support (<70). b Sequence logos for the predicted binding pocket residues in OlfC. Proximal sites predicted to be important for direct ligand “binding” are marked in black, distal binding sites responsible for binding “selectivity” are marked in gray, and the sites thought to be for structural maintenance are shown in blue. Note that the sites responsible for binding “selectivity” (marked in gray) showed much more variability in expanded OlfC subfamily 9 in ostariophysan fishes compared with other non-expanded OlfC subfamilies