Fig. 4

Arginine residues in M2[45–62] are more important for lamellipodium formation than are lysine residues. a Sequences of M2[45–62] and the M2-derivatives M2-R and M2-K. One-letter amino acid codes: R, arginine (Arg); L, leucine (Leu); F, phenylalanine (Phe); K, lysine (Lys); C, cysteine (Cys); I, isoleucine (Ile); Y, tyrosine (Tyr) and G, glycine (Gly). b Confocal laser scanning microscopy image of F-actin (stained with rhodamine–phalloidin) in COS-1 cells treated with 10 µm M2-R for 15 min. Scale bar, 20 µm. c Quantification of lamellipodium formation after treatment with M2[45–62] and the M2-derivatives (10 µm each). The mean ± standard error were derived from ~ 300 cells pooled from three independent experiments. *P < 0.05, Student’s t test