Fig. 5

M2[45–62] and M2-R decreased cell migration. a Migration of COS-1 cells treated with M2[45–62] and M2-R (10 µm) for 18 h at 37 °C. Migration length was calculated by measuring the speed and efficiency with which cells in a monolayer migrate to close a gap in the monolayer. Scale bar, 100 µm. b Average migration length of COS-1 cells treated with M2[45–62] or M2-R (10 µm each) in serum-free medium for 18 h at 37 °C under 5% CO₂, as described in the Supplementary Information. The average migration length during peptide treatment was calculated. c Relative transmigration of HeLa cells treated with M2[45–62] or M2-R (1 µm each) in serum-free medium for 6 h at 37 °C under 5% CO₂, as described in the Supplementary Information. The cells that migrated to the lower chamber were stained with 3’,6’-di(O-acetyl)-4’,5’-bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein, tetraacetoxymethyl ester (Calcein-AM). Relative signal intensities were calculated by comparing the fluorescence intensities of the peptide-treated and untreated cells. The fluorescence intensity was normalized to that of cells without peptide treatment. *P < 0.05, **P < 0.01, Student’s t test