Fig. 1

Histone status and repair cassette insertion sites. H4K16ac levels and DNA cleavage efficiency at the targeted human chromosome sites (described in Supplementary Figs. 1 and 3) in H1299, 293, and Hela cells. a Histone H4 levels at different sites with and without I-SceI induced DNA cut and with and without expression of dCas9-hMOF with gRNA in H1299 cells. The position of the sites is identified as Chr1A i.e., site A on chromosome 1 as shown in Supplementary Fig. 3. b H4K16ac levels at different native chromosome sites in exponentially growing cells were determined by ChIP/PCR and are represented as the relative levels. c The DR-GFP (for HR) or EJ5-GFP (for NHEJ) cassettes are inserted at the CRISPR-cleaved sites and a DSB is being induced by the expression of the I-SceI enzyme. GFP-positive cells resulting from HR and or NHEJ are measured by FACS. d H4K16ac levels at DR-GFP test genes inserted at different chromosome sites. e Western blot showing depletion of MOF with specific siRNA. f H4K16ac levels at different stages of the cell cycle