Fig. 4
From: Pre-existing H4K16ac levels in euchromatin drive DNA repair by homologous recombination in S-phase
Measurement of HR and repairosome protein recruitment to different genomic sites. a DR-GFP cassettes were inserted at different positions on the chromosome and the histograms present HR frequency at the sites. Chromosome sites designated as A, B, and C are Chr1A, Chr1B, Chr1C, Chr5A, Chr5B, Chr5C, Chr17A, and Chr17B. b Tagging of dCas-9-hMOF and gRNA at different sites increases the H4K16ac levels at all the sites. c Comparison of HR frequency at different sites among the cells with and without dCas-9-hMOF + gRNA. (d–k Detection of repair proteins by ChIP at different I-SceI sites before and after DSB induction. Cell synchronization, cell cycle analysis, I-SceI-induced DSB, and ChIP analysis were done according to the described procedure19,38,44. d Exponential cells for NHEJ proteins; e exponential cells for HR proteins; f G1 cells for NHEJ proteins; g G1 cells for HR proteins; h S cells for NHEJ proteins; i S cells for HR proteins; j G2/M cells for NHEJ proteins; k G2/M cells for HR proteins; l exponential cells for SMARCAD1 protein. (*P < 0.05; **P < 0.01 and ***P < 0.001, determined by the chi-square test)
