Fig. 6
From: Pre-existing H4K16ac levels in euchromatin drive DNA repair by homologous recombination in S-phase
Impact of RNA polymerase II and CSB on DSB repair. a Interaction between RNA polymerase II and CSB after cells being irradiated with 10 Gy in presence and absence of ATM inhibitor (KU55933) in H1299 cells. b Western blot showing CSB depletion at different times after transfection with specific siRNA. c CSB levels before and after induction of DSB at the different sites on chromosome 1. d Impact of CSB depletion on H4K16ac levels before and after DSB induction. e Impact of CSB depletion on RNA polymerase II loading at the DSB sites (A = Chr1A, B = Chr1B, and C = Chr1C). f Effect of CSB depletion on HR frequency at the different chromosomal locations (Site A = Chr1A, Chr5A, and Chr17A; Site B = Chr1B, Chr5B, and Chr17B). g Comparison of the relative frequency of DSB repair by NHEJ at the different sites on chromosome number 1 (Chr1A and Chr1B) with and without depletion of ligase IV. h Impact of α-Amanitin treatment on the recruitment of RNA polymerase II at the different sites DNA of chromosome 1 (A = Chr1A, B = Chr1B, and C = Chr1C) with and without induction of DSB. (*P < 0.05; **P < 0.01; ***P < 0.001 and ****P < 0.0001, determined by the chi-square test)
