Fig. 7

Loading of RNA polymerase II and CSB on RPA2 during repair. a I-SceI sites were introduced by CRISPR at the indicated positions in the human RPA2 gene (H1299 cells) using single-stranded oligonucleotides carrying the 18nt I-SceI site flanked with 45–90 bp homologous sequence. b H4K16ac levels at the different I-SceI sites of RPA2. c RNA polymerase II loading at site (i) of RPA2 after DSB induction. d Kinetics of SMARCAD1 association at I-SceI site of RPA2 (i) after DSB induction. e RNA polymerase II loading at three different I-SceI sites on RPA2 after induction of DSB. f CSB loading at the three different sites of RPA2 before and after induction of DSB. g Depletion of CSB impacts the loading of RNA polymerase II after induction of DSB at different sites of RPA2. h Recruitment of repairosome proteins at the DSB sites of RPA2 gene before and after induction of DSB. i MOF depletion by specific siRNA impacts the RNA polymerase II loading before and after induction of DSB at different sites of RPA2 gene. j MOF depletion by specific siRNA impacts the CSB loading before and after induction of DSB at different sites of RPA2 gene. k Association of repairosome protein recruitment to DSB sites after I-SceI expression in the RPA2 gene in cells depleted for MOF. (*P < 0.05; **P < 0.01; ***P < 0.001 and ****P < 0.0001, determined by the chi-square test)